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Determination of 4 psychoactive substances in tea using ultra high performance liquid chromatography combined with the quadrupole time-of-flight mass spectrometry

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Abstract

In this study, a method for the qualification and quantification of 4 psychoactive substances in tea using ultra high performance liquid chromatography coupled with the quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) has been developed. Tea samples were extracted by a 50% (v/v) methanol–water solution, and then separated by an ACQUITY UPLC BEH Shield RP18 column using a binary solvent system by gradient elution. The analytes were determined by Q-TOF/MS in TOFMS and information-dependent acquisition (IDA)-MS/MS mode. The results showed that the mass accuracy error of the 4 psychoactive substances were lower than 5.0 × 10−6, and a good linear relationship was observed in the range of 0.5–500 μg L−1 and correlation coefficient was higher than 0.9990. The LOD was in the range of 0.005–0.020 mg kg−1 and the LOQ was in the range of 0.010–0.040 mg kg−1. The recovery of the method was in range of 80.14–93.25% with spike levels of 0.010–0.400 mg kg−1, and relative standard deviations were lower than 10%. The method was simple, specific and reliable. It has been successfully used for the detection of 4 psychoactive substances in tea samples.

Graphical abstract: Determination of 4 psychoactive substances in tea using ultra high performance liquid chromatography combined with the quadrupole time-of-flight mass spectrometry

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Article information


Submitted
12 Aug 2020
Accepted
09 Sep 2020
First published
10 Sep 2020

Anal. Methods, 2020, Advance Article
Article type
Paper

Determination of 4 psychoactive substances in tea using ultra high performance liquid chromatography combined with the quadrupole time-of-flight mass spectrometry

J. Li, J. Ma, Y. Zhang and L. Zheng, Anal. Methods, 2020, Advance Article , DOI: 10.1039/D0AY01535K

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