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Development of an Unmodified Screen-Printed Graphene Electrode for Nonenzymatic Histamine Detection

Abstract

The high demand for food quality control has inspired the creation of high-performance sensing, cost-effectiveness and ease for use. Therefore, the aim of this work is to develop nonenzymatic electrochemical platforms for direct detection of histamine using an unmodified screen-printed graphene electrodes (SPGEs) for their applications such as evaluation of the fish freshness. In alkaline media (0.2M NaOH), unmodified SPGEs showed a very low oxidative potential of histamine at +0.58 V (vs. Ag/AgCl) avoiding perturbations from other biogenic amines. The developed method offers an excellent selectivity, sensitivity (the limit of detection (at 3SD/slope) of 0.62 mg/L) and wide working linear range (5-100 mg/L) for histamine detection. In addition, the proposed method was successfully applied to detect histamine in canned fish samples with recovery values ranging from 90.72% to 101.21%. Therefore, this newly proposed method is promising to be an alternative choice for the determination of histamine in fish samples and related food products.

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Supplementary files

Article information


Submitted
28 Jul 2020
Accepted
01 Oct 2020
First published
05 Oct 2020

Anal. Methods, 2020, Accepted Manuscript
Article type
Paper

Development of an Unmodified Screen-Printed Graphene Electrode for Nonenzymatic Histamine Detection

P. Nakthong, T. Kondo, O. Chailapakul and W. Siangproh, Anal. Methods, 2020, Accepted Manuscript , DOI: 10.1039/D0AY01443E

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