Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.



Simple and reliable analytical method for simultaneous quantification of first line antitubercular drugs in human plasma by LCMS/MS

Abstract

The present study describes the optimization of a simple and reliable method for the determination of four first line antitubercular drugs in human plasma. The studied analytes were Isoniazid (H), Rifampicin (R), Pyrazinamide (Z) and Ethambutol (E) in fixed dose combination recommended to patients under Revised National Tuberculosis Control Programme (RNTCP, India). The analytes were extracted from the human plasma (150 μL) using single step liquid-liquid extraction approach and were analyzed by liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS). The method was fully validated, according to USFDA guidelines. A linear range of 0.05–10 µg/ml, 0.1-20 µg/ml, 0.5-100 µg/ml and 0.05–10 µg/ml for H, R, Z and E respectively was established, presenting determination coefficients above 0.99. Concerning imprecision, the CV were lower than 15% for all analytes. All tested analytes were found to be stable in the samples. Although the values obtained for recovery were above 85%, the method proved to be sensitive, since low detection limits were 0.05 µg/ml for H and E, 0.1 µg/ml for R and 0.5 µg/ml for Z were obtained. The intra-day and inter-day accuracy and imprecision were within CV±15%. The use of conventional silica column in the extraction of these compounds through a single step protein precipitation method, simplify the analytical process. In addition due to its simplicity and sensitivity, it can be applied to carry out therapeutic drug monitoring and drug level assessment in human plasma samples. The results of analytes levels are comparable to other reported methods. The method had been successfully applied for simultaneous determination of first line anti-tubercular drugs in pulmonary tubercular patient. The method requires 150 µL of patient plasma, offers low volume for injection (10µL) and blood sample collection (3ml) which will be added advantage for pediatric anti-TB drug level assessment and monitoring.

Back to tab navigation

Article information


Submitted
02 May 2020
Accepted
28 Jun 2020
First published
29 Jun 2020

Anal. Methods, 2020, Accepted Manuscript
Article type
Paper

Simple and reliable analytical method for simultaneous quantification of first line antitubercular drugs in human plasma by LCMS/MS

B. K. Panda, M. Bargaje and S. L, Anal. Methods, 2020, Accepted Manuscript , DOI: 10.1039/D0AY00889C

Social activity

Search articles by author

Spotlight

Advertisements