Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.



Preparative purification of corilagin from Phyllanthus by combining ionic liquid extraction, prep-HPLC, and precipitation

Author affiliations

Abstract

In this study, a green extraction and purification process for the rapid preparation of corilagin from Phyllanthus has been designed using an aqueous ionic liquid coupled with preparative high-performance liquid chromatography (prep-HPLC) and precipitation. The results showed that the optimum extraction process for corilagin involved mixing Phyllanthus tenellus Roxb. with 0.4 M [BMIm]Br at a liquid–solid ratio of 10 : 1 and dispersing the mixture by ultrasonication at 50 °C for 15 min. Macroporous resin D101 and prep-HPLC were employed for [BMIm]Br removal and corilagin separation to yield corilagin of 86.49% purity. Subsequently, corilagin was further purified by water precipitation to achieve 99.12% purity. The results indicated the successful development of a new rapid and green process to prepare corilagin on a large scale from plants using [BMIm]Br. This promising process can be applied for the preparative separation and purification of other active compounds from complex plant systems.

Graphical abstract: Preparative purification of corilagin from Phyllanthus by combining ionic liquid extraction, prep-HPLC, and precipitation

Back to tab navigation

Supplementary files

Article information


Submitted
28 Apr 2020
Accepted
10 Jun 2020
First published
11 Jun 2020

Anal. Methods, 2020, Advance Article
Article type
Paper

Preparative purification of corilagin from Phyllanthus by combining ionic liquid extraction, prep-HPLC, and precipitation

X. Hou, Z. Cheng and J. Wang, Anal. Methods, 2020, Advance Article , DOI: 10.1039/D0AY00860E

Social activity

Search articles by author

Spotlight

Advertisements