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Colorimetric detection of class A soybean saponins by coupling DNAzyme with the gap ligase chain reaction

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Abstract

Class A saponins are responsible for the taste of soybean products, and the rapid identification of class A saponins from soybean food is essential for both food safety and cultivar screening. In this study, we propose a colorimetric assay based on the coupling of gap ligase chain reaction (Gap-LCR) with DNAzyme to detect the target GmSg-1 genes of class A soybean saponins with the naked eye, without the involvement of expensive instruments. The limits of detection (LODs) for the GmSg-1a and GmSg-1b genes were determined to be 0.1618 and 0.1625 μM, respectively, with a linear range of 0.2–1.2 μM. The DNAzyme-based Gap LCR assay was successfully employed to identify the target genes from different soybean cultivars, providing a simple means for monitoring the quality of soybean products.

Graphical abstract: Colorimetric detection of class A soybean saponins by coupling DNAzyme with the gap ligase chain reaction

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Article information


Submitted
21 Apr 2020
Accepted
19 May 2020
First published
22 May 2020

Anal. Methods, 2020, Advance Article
Article type
Paper

Colorimetric detection of class A soybean saponins by coupling DNAzyme with the gap ligase chain reaction

W. Li, G. Wu, M. Wang, A. Yue, W. Du, D. Liu and J. Zhao, Anal. Methods, 2020, Advance Article , DOI: 10.1039/D0AY00820F

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