Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.



Disposable biogenic amine biosensors for histamine determination in fish

Abstract

This study presents the development of disposable biosensors employed in the determination of histamine in fish sample. Screen printed carbon electrodes (SPCE) were first modified with a mixture of titanium dioxide nanoparticles (TiO2), carboxylated multiwalled carbon nanotubes (c-MWCNT), hexaammineruthenium (III) chloride (RU) and chitosan (CS). Diamine oxidase (DAO) or monoamine oxidase (MAO) enzymes were further immobilized onto TiO2−c-MWCNT−RU−CS/SPCE via 1‐ethyl‐3‐(dimethylaminopropyl) carbodiimidehydrochloride (EDC) and hydroxysuccinimide (NHS) chemistry for the fabrication of the biosensors. The morphological and electrochemical properties of the proposed biosensors were studied using scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), cyclic voltammetry (CV), chronoamperometry and electrochemical impedance spectroscopy (EIS). A performance comparison of two biosensors indicated that the one based on DAO had a linear concentration range from 9.9×10-6 – 1.1×10-3 M and the other based on MAO from 5.6×10-5–1.1×10-3 M for histamine. The sensitivity of the DAO based biosensor was almost 1.5 times higher than that of the MAO based biosensor. The proposed biosensors were successfully employed to determine histamine in fish sample and the recoveries were between 100.0% and 104.6%.

Back to tab navigation

Supplementary files

Article information


Submitted
19 Apr 2020
Accepted
21 Jun 2020
First published
24 Jun 2020

Anal. Methods, 2020, Accepted Manuscript
Article type
Paper

Disposable biogenic amine biosensors for histamine determination in fish

İ. Okman Koçoğlu, P. E. Erden and E. Kilic, Anal. Methods, 2020, Accepted Manuscript , DOI: 10.1039/D0AY00802H

Social activity

Search articles by author

Spotlight

Advertisements