Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 25, 2020
Previous Article Next Article

The sensitive detection of single-cell secreted lactic acid for glycolytic inhibitor screening with a microdroplet biosensor

Author affiliations

Abstract

Lactic acid (LA) plays an important role in the tumor metabolism and malignant progression of various cancers. Herein, we have developed a one-step, wash-free microfluidic approach with droplet biosensors for the sensitive detection of LA secreted by a single tumor cell. Our assay integrates the enzyme-assisted chemical conversion of LA in small-volume (4.2 nL) droplets for fluorescence signal readout. The microdroplet assay achieved a limit of detection of 1.02 μM and was more sensitive than the commercial ELISA kit by nearly two orders of magnitude. A good specificity has been demonstrated for this assay by testing various ions and biomolecules from the culture medium. This droplet assay allows us to acquire the profiles of the lactic acid secretion of tumor cells under the influence of glycolytic inhibitors at the single-cell level. It offers a useful research tool to study the cell-to-cell differences of LA secretion and glycolytic inhibitor screening for cancer research.

Graphical abstract: The sensitive detection of single-cell secreted lactic acid for glycolytic inhibitor screening with a microdroplet biosensor

Back to tab navigation

Supplementary files

Article information


Submitted
27 Mar 2020
Accepted
21 May 2020
First published
26 May 2020

Anal. Methods, 2020,12, 3250-3259
Article type
Paper

The sensitive detection of single-cell secreted lactic acid for glycolytic inhibitor screening with a microdroplet biosensor

X. Chen, R. Shen, S. Liu, X. Xiao, J. Yan, Y. Zhang, Z. Jiang, B. Nie and J. Liu, Anal. Methods, 2020, 12, 3250
DOI: 10.1039/D0AY00633E

Social activity

Search articles by author

Spotlight

Advertisements