Fast magnetic solid-phase extraction using Fe3O4-NH2@MOF material for monohydroxy polycyclic aromatic hydrocarbons in urine of coke-oven workers
In this work, magnetic material (Fe3O4-NH2@MIL-101) was successfully prepared, and the material was used as a sorbent for the magnetic solid-phase extraction (MSPE) of trace level monohydroxy polycyclic aromatic hydrocarbons (OH-PAHs) in urine samples for the first time. The target analytes were quantified by high performance liquid chromatography equipped with fluorescence detection (HPLC-FLD). The MSPE key factors, including the amount of adsorbent, extraction time, pH, the effect of salt, eluting solutions and eluant volume were systematically optimized. Under the optimized conditions, the developed method showed good linearity (0.03-200 ng/mL), low limits of detection (0.016-0.042 ng/mL, signal-to-noise ratio = 3) and satisfactory repeatability (relative standard deviation ≤ 10.1%; n = 5). The method showed stable average recoveries ranging from 78.3% to 112.9% and the enrichment factors were 9 to 15. Besides the satisfying method parameters, the total MPSE process could be completed with no more than 5 minutes. This results indicated that the Fe3O4-NH2@MOF based MSPE was a simple, efficient and fast method which was suitable for MSPE of OH-PAHs from urine samples.