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Perfluorosulfonic acid polymer based eATRP for ultrasensitive detection of CYFRA21-1 DNA

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Abstract

The sensitive detection of biomarker cytokeratin fragment antigen 21-1 (CYFRA21-1) is crucial for early diagnosis and screening of non-small cell lung cancer (NSCLC). In this work, an electrochemical biosensor based on Nafion-initiated eATRP has been built for ultrasensitive detection of CYFRA21-1 DNA for the first time. Specifically, peptide nucleic acid (PNA) probes are immobilized onto a gold electrode surface and then hybridized with target DNA to form PNA/DNA heteroduplexes for the subsequent attachment of Nafion by the identified carboxyl–Zr4+–phosphoric acid chemistry. Finally, polymer chains are obtained by linking the monomer of ferrocenylmethyl methacrylate to the PNA/MCH/DNA/Zr4+/Nafion probes via eATRP. Under optimized steady-state conditions, the sensor offers a wide current response for CYFRA21-1 DNA from 10−11 to 10−16 M with a detection limit of 6.42 × 10−17 M. The proposed method of using Nafion as the eATRP initiator exhibits high sensitivity, reproducibility and stability and is a promising strategy for early diagnosis of NSCLC.

Graphical abstract: Perfluorosulfonic acid polymer based eATRP for ultrasensitive detection of CYFRA21-1 DNA

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Supplementary files

Article information


Submitted
15 Feb 2020
Accepted
11 May 2020
First published
12 May 2020

Anal. Methods, 2020, Advance Article
Article type
Paper

Perfluorosulfonic acid polymer based eATRP for ultrasensitive detection of CYFRA21-1 DNA

K. Hu, J. Cheng, J. Li, S. Ye, H. Yang, Y. Liu and J. Kong, Anal. Methods, 2020, Advance Article , DOI: 10.1039/D0AY00328J

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