Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.



An aptasensor for the detection of ampicillin in milk using a personal glucose meter

Author affiliations

Abstract

Antibiotic residues in foods have aroused wide public concern because of their potential side-effects. It is imperative to develop a simple, accurate and reliable method for the detection of antibiotic residues in foods. In this paper, we report a novel, facile and sensitive method for the detection of ampicillin in milk using a commercial personal glucose meter (PGM). Magnetic beads (MBs) were employed as the platform, an ampicillin aptamer was used as the recognition element and streptavidin was utilized as the bridge to link invertase and the aptamer. After the hydrolysis of sucrose to glucose, the concentration of glucose was quantitatively measured using the PGM. The difference of PGM signals with and without addition of ampicillin exhibits a good linear correlation with the logarithm of ampicillin concentrations in the range of 2.5 × 10−10 mol L−1 to 1.0 × 10−7 mol L−1 with a detection limit of 2.5 × 10−10 mol L−1 (S/N = 3). Finally, the proposed method was successfully applied for the detection of ampicillin residue in milk.

Graphical abstract: An aptasensor for the detection of ampicillin in milk using a personal glucose meter

Back to tab navigation

Article information


Submitted
07 Feb 2020
Accepted
25 May 2020
First published
26 May 2020

Anal. Methods, 2020, Advance Article
Article type
Paper

An aptasensor for the detection of ampicillin in milk using a personal glucose meter

F. Li, X. Li, N. Zhu, R. Li, H. Kang and Q. Zhang, Anal. Methods, 2020, Advance Article , DOI: 10.1039/D0AY00256A

Social activity

Search articles by author

Spotlight

Advertisements