Droplet image analysis with user-friendly freeware CellProfiler
Droplet microfluidic assays are rapidly gaining popularity because they enable analyzing biochemical reactions, individual cells or small cell populations with high sensitivity, precision and accuracy in a high-throughput manner. Nonetheless, there is a demand for user-friendly and low-cost droplet analysis technology. In this article, we meet this demand by developing two droplet analysis pipelines via free open-source software CellProfiler (CP) and its companion (CellProfiler Analyst, CPA). To illustrate the competence of the pipelines as an independent, and freely accessible droplet analysis tool for any researcher without the need of programming skills, we show i) droplet digital quantification of viable fluorescent bacteria using single-color image and ii) analysis of a multi-color fluorescence image with droplets containing different chemical compositions.