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Droplet image analysis with user-friendly freeware CellProfiler

Abstract

Droplet microfluidic assays are rapidly gaining popularity because they enable analyzing biochemical reactions, individual cells or small cell populations with high sensitivity, precision and accuracy in a high-throughput manner. Nonetheless, there is a demand for user-friendly and low-cost droplet analysis technology. In this article, we meet this demand by developing two droplet analysis pipelines via free open-source software CellProfiler (CP) and its companion (CellProfiler Analyst, CPA). To illustrate the competence of the pipelines as an independent, and freely accessible droplet analysis tool for any researcher without the need of programming skills, we show i) droplet digital quantification of viable fluorescent bacteria using single-color image and ii) analysis of a multi-color fluorescence image with droplets containing different chemical compositions.

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Supplementary files

Article information


Submitted
06 Jan 2020
Accepted
25 Mar 2020
First published
25 Mar 2020

Anal. Methods, 2020, Accepted Manuscript
Article type
Technical Note

Droplet image analysis with user-friendly freeware CellProfiler

S. Bartkova, M. Vendelin, I. Sanka, P. Pata and O. Scheler, Anal. Methods, 2020, Accepted Manuscript , DOI: 10.1039/D0AY00031K

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