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A sensitive and selective fluorescent probe for the detection of endogenous peroxynitrite (ONOO) in living cells

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Abstract

Peroxynitrite (ONOO) is one of reactive oxygen species, and plays a vital role in numeorus physiological and pathological processes. Given that the ONOO level is closely related with various serious diseases, the in situ and real time detection of endogenous ONOO is highly important for the in-depth study of its roles in living systems. Herein, we present a new fluorescent probe (RHPN) for the real-time detection of intracellular ONOO. The probe RHPN consists of a rhodamine analogue and an arylhydrazide group as a response site for ONOO. In response to ONOO, the probe RHPN converts to an open-ring form and generates strong fluorescence. Moreover, the probe RHPN was successfully used for the imaging of the endogenous and exogenous ONOO level changes in living cells.

Graphical abstract: A sensitive and selective fluorescent probe for the detection of endogenous peroxynitrite (ONOO−) in living cells

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Supplementary files

Article information


Submitted
03 Jan 2020
Accepted
06 May 2020
First published
07 May 2020

Anal. Methods, 2020, Advance Article
Article type
Paper

A sensitive and selective fluorescent probe for the detection of endogenous peroxynitrite (ONOO) in living cells

Y. Sun, B. Dong, Y. Lu, W. Song, A. H. Mehmood and W. Lin, Anal. Methods, 2020, Advance Article , DOI: 10.1039/D0AY00012D

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