A novel microfluidic paper-based analytical device based on chemiluminescence for determination of β-agonists in Swine Hair
β-agonists are illegal feed additives in feed industry for many countries, especially in China. Here, we report a microfluidic paper-based analytical device (µPAD) coupled with chemiluminescence (CL) method to provide sensitive, simple and rapid quantitative measurement of β-agonists in swine hair samples. In this study, we found that the β-agonists diminished the CL generated by the reaction of K3[Fe (CN)6] and luminol on the µPAD, which is different from that in the aqueous solution, and the degree of diminishment was proportional to the concentration of β-agonists. The possible mechanism was discussed as well. And this detection method showed wide linear range ( from 4.0×10-8 to 1.0×10-5 mol/L) and low limit of detection (2.0×10-8mol/L) with low samples and reagents consumption. Satisfactory recovery values (from 78% to 95%) were achieved. Therefore, our µPAD CL sensor will be favorable to develop miniaturization instrument for on-site analysis of β-agonists in swine hair samples.