Designing logic gates based on 3-Way DNAzyme complex
In recent years, DNA has been regarded as a reliable raw material for building biological computers and biochips due to its nanoscale size, ultralow energy consumption, and high-performance computing potential. As the basis of building a biological computer, the research on the construction of nanoscale logic arithmetic and nanoscale biochemical logic circuits based on DNA molecules as carriers has attracted increasing attention. Since DNA molecules pair up with their own bases to form a secondary structure in the liquid phase, and the logic circuit thus formed has a single function. How to enhance the cascade effect and reusability of DNA molecules has become an urgent problem. To solve this problem, we proposed a 3-way DNAzyme complex composed of three E6 DNAzymes by combining ssDNA and the biological characteristics of E6 DNAzyme. This complex enriched the recognition vector of E6 DNAzyme, which can be used for ssDNA multiple times, thus improving the reusability and efficiency of DNA molecules. At the same time, based on the 3-way DNAzyme complex and using ssDNA as the input and output signals, we designed logic gates such as the OR gate, the AND gate, and the INHIBIT gate, and realized the construction of a new half subtractor and nanoscale biochemical logic circuit that further enriched the cascade effect of DNA molecules. We believe that these logic elements will have a wide range of applications in DNA nanoscale programming, biological computing, and nanoscale medicine.