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Application of electrochemical LAMP-based assay for screening of HPV16/HPV18 infection in cervical samples

Abstract

Cervical cancer is caused by a persistent infection with high-risk types of human papillomaviruses (HPV), especially HPV16 and HPV18. HPV testing therefore represents an important alternative to standard cytological screening. However, existing HPV tests are often laborious, expensive or time-consuming, and thus novel techniques are developed that would be inexpensive, rapid, easy to perform and could be implemented in low-resource settings. In this work, we show an early application of an electrochemical (EC) assay combined with loop-mediated amplification (LAMP) to determine HPV16 and HPV18 status directly in clinical material. To demonstrate its potential usefulness, we screened 61 cervical samples for presence of both genotypes, and compared the results with golden standard - PCR amplification followed by gel electrophoresis, as well as with two commercial HPV tests - COBAS and INNO-LiPA. The best concordance was obtained with PCR reaction; we reached high significance (p-value <0.0001) and positive and negative predictive values over 90%. These data indicate that EC-LAMP assay may serve as an interesting tool for rapid screening of oncogenic HPVs.

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Article information


Submitted
04 Nov 2019
Accepted
22 Dec 2019
First published
02 Jan 2020

Anal. Methods, 2020, Accepted Manuscript
Article type
Paper

Application of electrochemical LAMP-based assay for screening of HPV16/HPV18 infection in cervical samples

M. Anton, L. J. Moranova, R. Hrstka and M. Bartošík, Anal. Methods, 2020, Accepted Manuscript , DOI: 10.1039/C9AY02383F

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