Issue 7, 2020

Recent advances in fluorescence resonance energy transfer-based probes in nucleic acid diagnosis

Abstract

Nucleic acid diagnosis is a method that diagnoses human conditions and diseases by directly exploring the existing states or defects of nucleic acids using theoretical and technical approaches from molecular biology. The detected genes are either endogenous (the body's own genes) or exogenous (such as viruses and bacteria). The former is used to diagnose genetic lesions and the latter is used to diagnose pathogenic infections. Fluorescent hybridization probes based on the principles of fluorescence resonance energy transfer (FRET) and the complementary pairing of nucleic acids have been widely used in nucleic acid detection due to their high sensitivity and selectivity, ease of design and synthesis. These probes have been extensively studied and modified for different applications. Traditional fluorescent hybridization probes include molecular beacons (MBs) and binary probes (BPs). Many new fluorescent hybridization probes have been derived to improve the sensitivity of the traditional probes by adjusting their structure, combined with amplification techniques and novel nanomaterials. This review describes the applicability and advantages of various hybridization probes that utilize novel and inventive designs.

Graphical abstract: Recent advances in fluorescence resonance energy transfer-based probes in nucleic acid diagnosis

Article information

Article type
Minireview
Submitted
29 Oct 2019
Accepted
17 Jan 2020
First published
31 Jan 2020

Anal. Methods, 2020,12, 884-893

Recent advances in fluorescence resonance energy transfer-based probes in nucleic acid diagnosis

J. Chen, C. Shi, X. Y. Kang, X. T. Shen, X. Lao and H. Zheng, Anal. Methods, 2020, 12, 884 DOI: 10.1039/C9AY02332A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements