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Issue 7, 2020
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A novel signal amplification strategy for highly specific and nonenzymatic isothermal electrochemiluminescence detection of tumour markers

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Abstract

The small size of microRNAs in biofluids presents challenges for detection by quantitative real-time polymerase chain reaction (qRT-PCR). In this work, a sensitive electrochemiluminescent probe based on dendritic DNA and streptavidin-coated magnetic beads was fabricated, and good amplification efficiency and sample loading efficiency were obtained. Thus a nonenzymatic and isothermal electrochemiluminescence method was proposed for the sensitive detection of miRNA-21. The limit of detection was as low as 4 fmol, which was superior to that for the reported linear DNA method. In real tumor cell analysis, the proposed method achieved equivalent detection results to the qRT-PCR method, guaranteeing the validity and feasibility of routine inspection.

Graphical abstract: A novel signal amplification strategy for highly specific and nonenzymatic isothermal electrochemiluminescence detection of tumour markers

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Supplementary files

Article information


Submitted
26 Oct 2019
Accepted
22 Jan 2020
First published
27 Jan 2020

Anal. Methods, 2020,12, 938-942
Article type
Paper

A novel signal amplification strategy for highly specific and nonenzymatic isothermal electrochemiluminescence detection of tumour markers

Y. Liu, X. Guo, Z. Fan, Y. Liao, Y. Yu, M. Guo, Y. Cao and D. Zhu, Anal. Methods, 2020, 12, 938
DOI: 10.1039/C9AY02310K

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