Jump to main content
Jump to site search


A quadruple-label time-resolved fluorescence immunochromatographic assay for simultaneous quantitative determination of three mycotoxins in grains

Author affiliations

Abstract

A multiplex quadruple-label time-resolved fluorescence immunochromatographic assay (QL-TRFICA) for simultaneous quantitative detection of aflatoxin B1 (AFB1), zearalenone (ZEN) and ochratoxin A (OTA) in grains was established in this paper. This assay was developed based on quadruple-label immunoprobes, one of which was a chicken IgY-specific antibody coupled with time-resolved fluorescent nanobeads (TRFN) for the test C line signal while the other three were specific to AFB1, ZEN and OTA monoclonal antibodies coupled with TRFN for the test T line signal. Quantitative relationships between the concentrations of the three mycotoxins and T/C ratios were established to determine the analyte concentration through a portable multi-channel fluorescence reader. The limits of detection (LODs) for AFB1, ZEN and OTA were 0.04 μg kg−1, 0.20 μg kg−1 and 0.10 μg kg−1 in 6 grains (corn, soybean, sorghum, wheat, rice and oats), respectively. Recoveries ranged from 71.60% to 119.98% at fortified concentrations of LOD, 2LOD and 4LOD, with a coefficient of variation <15%. Analysis of field grain samples by QL-TRFICA was in accordance with that of LC-MS/MS. Therefore, this proposed multiplex QL-TRFICA was a promising method for on-site screening of multiple mycotoxins in grains.

Graphical abstract: A quadruple-label time-resolved fluorescence immunochromatographic assay for simultaneous quantitative determination of three mycotoxins in grains

Back to tab navigation

Supplementary files

Article information


Submitted
26 Oct 2019
Accepted
27 Nov 2019
First published
27 Nov 2019

Anal. Methods, 2020, Advance Article
Article type
Paper

A quadruple-label time-resolved fluorescence immunochromatographic assay for simultaneous quantitative determination of three mycotoxins in grains

X. Chang, Y. Zhang, H. Liu and X. Tao, Anal. Methods, 2020, Advance Article , DOI: 10.1039/C9AY02309G

Social activity

Search articles by author

Spotlight

Advertisements