Jump to main content
Jump to site search


Development of an electrochemical aptasensor using Au octahedra and graphene for signal amplification

Author affiliations

Abstract

A novel electrochemical aptasensor was constructed for ochratoxin A (OTA) detection based on Au octahedra and graphene (GR) as a signal amplification strategy. Au octahedra with eight triangle faces and sharp corners were prepared and acted as carriers to connect more thionine (Th) and complementary DNA (cDNA) to form Th–Au octahedra–cDNA. Th–Au octahedra–dsDNA was formed by hybridizing OTA aptamers with cDNA. GR with high surface area and good conductivity immobilized more biomolecules and facilitated electron transfer on the electrode surface. Th–Au octahedra–dsDNA was immobilized on the GR/GCE surface, producing a large initial peak current. In the presence of OTA, the formation of the aptamer–OTA complex induced the release of Th–Au octahedra–cDNA from the electrode surface and decreased the electrochemical signal of Th. The proposed aptasensor exhibited a wider linear range from 0.001 to 5 ng mL−1 with a lower detection limit of 0.13 pg mL−1; it also showed good selectivity against common interferences, including AFB1, ZEA and FB1.

Graphical abstract: Development of an electrochemical aptasensor using Au octahedra and graphene for signal amplification

Back to tab navigation

Article information


Submitted
12 Sep 2019
Accepted
02 Dec 2019
First published
03 Dec 2019

Anal. Methods, 2020, Advance Article
Article type
Paper

Development of an electrochemical aptasensor using Au octahedra and graphene for signal amplification

M. Wei, S. Yue, W. Zhang and X. Li, Anal. Methods, 2020, Advance Article , DOI: 10.1039/C9AY01981B

Social activity

Search articles by author

Spotlight

Advertisements