A new approach to study human perivascular adipose tissue of internal mammary artery by fiber optic Raman spectroscopy supported by spectral modelling
Perivascular adipose tissue (PVAT) regulates vascular function and represents a novel therapeutic target in vascular diseases. In this work, a new approach, based on fiber optic Raman spectroscopy and spectral modelling was used to the characterize chemical content of PVAT of internal mammary artery (IMA) of patients with advanced coronary atherosclerosis (n=10) undergoing coronary bypass surgery. Our results showed a high degree of lipid unsaturation and low carotenoid content in PVAT of IMA in patients with more advanced coronary artery disease. Moreover, the spectral modelling of the IMA’s PVAT composition indicated that glyceryl trioleate was a major PVAT lipid and for patients with relatively low levels of -carotene was accompanied by arachidonic acid and glyceryl trilinolenate. In summary, our proof-of-concept study suggests that the carotenoid content and lipid unsaturation degree may reflect PVAT functional status and Raman-based assessment of PVAT of IMA could prove useful as a novel diagnostic tool to rapidly define the phenotype of PVAT in a grafted artery in patients undergoing coronary bypass.