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Photon-upconversion barcode for monitoring an enzymatic reaction with a fluorescence reporter in droplet microfluidics

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Abstract

We report luminescent photon-upconversion barcodes for indexing the chemical content of droplets. The barcode is compatible with the simultaneous detection of fluorescence. The encoding and decoding of the initial concentration of enzyme β-galactosidase and substrate 4-methylumbelliferyl β-D-galactopyranoside are described. The fluorescent product 4-methylumbelliferone is detected simultaneously with the barcode.

Graphical abstract: Photon-upconversion barcode for monitoring an enzymatic reaction with a fluorescence reporter in droplet microfluidics

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Article information


Submitted
18 Aug 2020
Accepted
22 Sep 2020
First published
23 Sep 2020

Analyst, 2020, Advance Article
Article type
Paper

Photon-upconversion barcode for monitoring an enzymatic reaction with a fluorescence reporter in droplet microfluidics

A. Hlaváček, J. Křivánková, N. Pizúrová, T. Václavek and F. Foret, Analyst, 2020, Advance Article , DOI: 10.1039/D0AN01667E

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