Issue 23, 2020

Photon-upconversion barcode for monitoring an enzymatic reaction with a fluorescence reporter in droplet microfluidics

Abstract

We report luminescent photon-upconversion barcodes for indexing the chemical content of droplets. The barcode is compatible with the simultaneous detection of fluorescence. The encoding and decoding of the initial concentration of enzyme β-galactosidase and substrate 4-methylumbelliferyl β-D-galactopyranoside are described. The fluorescent product 4-methylumbelliferone is detected simultaneously with the barcode.

Graphical abstract: Photon-upconversion barcode for monitoring an enzymatic reaction with a fluorescence reporter in droplet microfluidics

Supplementary files

Article information

Article type
Paper
Submitted
18 Aug 2020
Accepted
22 Sep 2020
First published
23 Sep 2020

Analyst, 2020,145, 7718-7723

Photon-upconversion barcode for monitoring an enzymatic reaction with a fluorescence reporter in droplet microfluidics

A. Hlaváček, J. Křivánková, N. Pizúrová, T. Václavek and F. Foret, Analyst, 2020, 145, 7718 DOI: 10.1039/D0AN01667E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements