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Issue 24, 2020
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A three-dimensional CoNi-MOF nanosheet array-based immunosensor for sensitive monitoring of human chorionic gonadotropin with core–shell ZnNi-MOF@Nile Blue nanotags

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Abstract

A CoNi-based metal–organic framework (CoNi-MOF) nanosheet array is synthesized by the treatment of a CoNi layered double hydroxide nanosheet array on Ni foam with 3,5-diaminobenzoic acid. The CoNi-MOF nanosheet array with amino and carboxyl groups can be used to capture the human chorionic gonadotropin (HCG) primary antibody (HCG Ab1). Nile Blue decorated ZnNi-MOF (NB@ZnNi-MOF) spheres immobilized with HCG secondary antibodies (HCG Ab2) are used for signal amplification. When HCG exists in an analytical sample, a sandwich structure is formed and an electrochemical signal is produced. The analytical signal generated during the detection is caused by the conversion of Co(II) and Co(III) in the CoNi-MOF nanosheet array. The Nile Blue of the NB@ZnNi-MOF sphere, as a kind of redox-active species, is responsible for the electrochemical signal amplification in the immunosensor. On the basis of the above advantages, the HCG immunosensor exhibits a lower limit of detection (1.85 × 10−3 mIU mL−1) and a wide linear range from 0.005 mIU mL−1 to 250 mIU mL−1. Additionally, this immunosensor is used to quantitatively detect HCG in human blood serum and shows good correlations with the standard enzyme-linked immunosorbent assay (ELISA), providing a high value on clinical diagnosis.

Graphical abstract: A three-dimensional CoNi-MOF nanosheet array-based immunosensor for sensitive monitoring of human chorionic gonadotropin with core–shell ZnNi-MOF@Nile Blue nanotags

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Supplementary files

Article information


Submitted
15 Aug 2020
Accepted
21 Sep 2020
First published
22 Sep 2020

Analyst, 2020,145, 8097-8103
Article type
Paper

A three-dimensional CoNi-MOF nanosheet array-based immunosensor for sensitive monitoring of human chorionic gonadotropin with core–shell ZnNi-MOF@Nile Blue nanotags

W. Lu, Z. Chen, M. Wei, X. Cao and X. Sun, Analyst, 2020, 145, 8097
DOI: 10.1039/D0AN01648A

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