Jump to main content
Jump to site search

Issue 23, 2020
Previous Article Next Article

Detection of the SARS-CoV-2 humanized antibody with paper-based ELISA

Author affiliations

Abstract

This work reports the development of a rapid, simple and inexpensive colorimetric paper-based assay for the detection of the severe acute respiratory symptom coronavirus 2 (SARS-CoV-2) humanized antibody. The paper device was prepared with lamination for easy sample handling and coated with the recombinant SARS-CoV-2 nucleocapsid antigen. This assay employed a colorimetric reaction, which is followed by horseradish peroxidase (HRP) conjugated detecting antibody in the presence of the 3,3′,5,5′-tetramethylbenzidine (TMB) substrate. The colorimetric readout was evaluated and quantified for specificity and sensitivity. The characterization of this assay includes determining the linear regression curve, the limit of detection (LOD), the repeatability, and testing complex biological samples. We found that the LOD of the assay was 9.00 ng μL−1 (0.112 IU mL−1). The relative standard deviation was approximately 10% for a sample number of n = 3. We believe that our proof-of-concept assay has the potential to be developed for clinical screening of the SARS-CoV-2 humanized antibody as a tool to confirm infected active cases or to confirm SARS-CoV-2 immune cases during the process of vaccine development.

Graphical abstract: Detection of the SARS-CoV-2 humanized antibody with paper-based ELISA

Back to tab navigation

Supplementary files

Article information


Submitted
11 Aug 2020
Accepted
31 Aug 2020
First published
01 Sep 2020

Analyst, 2020,145, 7680-7686
Article type
Paper

Detection of the SARS-CoV-2 humanized antibody with paper-based ELISA

S. Kasetsirikul, M. Umer, N. Soda, K. R. Sreejith, M. J. A. Shiddiky and N. Nguyen, Analyst, 2020, 145, 7680
DOI: 10.1039/D0AN01609H

Social activity

Search articles by author

Spotlight

Advertisements