Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 21st October 2020 from 07:00 AM to 07:00 PM (BST).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.



Rapid and sensitive electrochemical detection of microRNAs by gold nanoparticle-catalyzed silver enhancement

Author affiliations

Abstract

MicroRNAs (miRNAs) have played a vital role in the regulation of gene expression and have been considered as potential biomarker candidates for early cancer diagnosis. Rapid and sensitive detection of microRNAs is highly desired. Here, we present a new method to rapidly and sensitively determine microRNAs based on the technology of gold nanoparticle catalyzed silver staining enhancement. The new method involves the sandwich hybridization of a capture probe immobilized on a magnetic bead, a reporter probe assembled on gold nanoparticles and a miRNA target, catalytic silver precipitation by gold nanoparticles, magnetic collection of the enhanced sandwich complex, dissolution of the silver precipitation and stripping detection. Using the proposed method the microRNA-7a assay was successfully carried out in less than 70 min and the detection limit was as low as 15 fM. The proposed biosensor may hold great promise in biological monitoring of microRNAs.

Graphical abstract: Rapid and sensitive electrochemical detection of microRNAs by gold nanoparticle-catalyzed silver enhancement

Back to tab navigation

Article information


Submitted
10 Aug 2020
Accepted
27 Aug 2020
First published
02 Sep 2020

Analyst, 2020, Advance Article
Article type
Paper

Rapid and sensitive electrochemical detection of microRNAs by gold nanoparticle-catalyzed silver enhancement

Q. Wen, X. Liang, H. Pan, J. Li, Y. Zhang, W. Zhu and Z. Long, Analyst, 2020, Advance Article , DOI: 10.1039/D0AN01606C

Social activity

Search articles by author

Spotlight

Advertisements