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Fe–N/C single-atom nanozyme-based colorimetric sensor array for discriminating multiple biological antioxidants

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Abstract

Identifying the species and concentrations of antioxidants is really important because antioxidants play important roles in various biological processes and numerous diseases. Compared with an individual sensor detecting a single antioxidant with limited specificity, a sensor array could simultaneously identify various antioxidants, in which 3–5 types of nanomaterials with peroxidase-like activity are absolutely necessary. Herein, as a single-atom nanozyme, Fe–N/C with oxidase-mimicking activity was applied to construct a triple-channel colorimetric sensor array: (1) Fe–N/C catalytically oxidized three substrates 3,3′,5,5′-tetramethylbenzidine (TMB), 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and o-phenylenediamine (OPD) to produce blue oxidized TMB (oxTMB), green oxidized ABTS (oxABTS) and yellow oxidized OPD (oxOPD), respectively; (2) with oxTMB, oxABTS and oxOPD as three sensing channels, a colorimetric sensor array was constructed for simultaneously discriminating glutathione (GSH), L-cysteine (L-Cys), ascorbic acid (AA), uric acid (UA), and melatonin (MT), even quantifying concentrations (with GSH as a model analyst). The performance of the sensor array was validated through accurately identifying 15 blind samples containing GSH, L-Cys, AA, UA and MT in buffer solution and human serum samples, and also in binary and ternary mixtures. This work proved that fabricating a single nanozyme-based sensor array was a simplified and reliable strategy for simultaneously probing multiple antioxidants.

Graphical abstract: Fe–N/C single-atom nanozyme-based colorimetric sensor array for discriminating multiple biological antioxidants

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Supplementary files

Article information


Submitted
21 Jul 2020
Accepted
26 Sep 2020
First published
28 Sep 2020

Analyst, 2020, Advance Article
Article type
Paper

Fe–N/C single-atom nanozyme-based colorimetric sensor array for discriminating multiple biological antioxidants

W. Jing, X. Cui, F. Kong, W. Wei, Y. Li, L. Fan and X. Li, Analyst, 2020, Advance Article , DOI: 10.1039/D0AN01447H

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