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Thread-based isoelectric focusing coupled with desorption electrospray ionization mass spectrometry

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Abstract

The combination of a thread-based electrofluidic analytical device and desorption electrospray ionization mass-spectrometry (DESI-MS) was investigated for the separation and concentration of proteins. The combination delivered a low-cost novel approach for sample pretreatment and target focusing, with direct “on-thread” ambient mass spectrometry detection. For this purpose, a platform for thread-based isoelectric focusing (TB-IEF) was 3D-printed, optimised, and applied to the separation and focusing of three model proteins. Successful separation and focusing was achieved within 30 min. The TB-IEF device was coupled with DESI-MS by direct exposure of the focused solutes on the dried thread to the DESI source. As a proof-of-concept, a 10-fold increase in the DESI-MS response for insulin was achieved following the TB-IEF preconcentration, whilst simultaneously isolating the target solutes from their sample matrix.

Graphical abstract: Thread-based isoelectric focusing coupled with desorption electrospray ionization mass spectrometry

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Supplementary files

Article information


Submitted
06 Jul 2020
Accepted
18 Aug 2020
First published
18 Aug 2020

Analyst, 2020, Advance Article
Article type
Paper

Thread-based isoelectric focusing coupled with desorption electrospray ionization mass spectrometry

L. Chen, J. M. Cabot, E. Sanz Rodriguez, A. Ghiasvand, P. C. Innis and B. Paull, Analyst, 2020, Advance Article , DOI: 10.1039/D0AN01344G

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