Jump to main content
Jump to site search


A spiropyran functionalized fluorescent probe for mitochondria targeting and imaging of endogenous hydrogen sulfide in living cells

Author affiliations

Abstract

A turn-on spiropyran functionalized fluorescein derivative (FMC) is developed for targeting HS in mitochondria. FMC exhibits very weak fluorescence at 525 nm under the excitation of 470 nm in aqueous solution due to its colorless spiropyran form; upon addition of HS, a strong fluorescence enhancement by 6.4-fold is observed with spirocycle-opened merocyanine form and rapid trapping kinetics for HS. FMC has good biocompatibility and high selectivity towards HS with a detection limit of 88.2 nM and is very sensitive among the reported H2S fluorescent probes. Moreover, the significant colocalization of FMC with Mito Tracker® Deep Red FM in human laryngeal epidermoid carcinoma (HEp-2) cells and the Pearson correlation coefficient of 0.87 together demonstrate that FMC can target and image the endogenous H2S in the mitochondria of living cells.

Graphical abstract: A spiropyran functionalized fluorescent probe for mitochondria targeting and imaging of endogenous hydrogen sulfide in living cells

Back to tab navigation

Supplementary files

Article information


Submitted
30 Jun 2020
Accepted
21 Sep 2020
First published
21 Sep 2020

Analyst, 2020, Advance Article
Article type
Paper

A spiropyran functionalized fluorescent probe for mitochondria targeting and imaging of endogenous hydrogen sulfide in living cells

Q. Liu, Y. Zhao, Y. Zhang, K. Xie, R. Liu, B. Ren, Y. Yan and L. Li, Analyst, 2020, Advance Article , DOI: 10.1039/D0AN01298J

Social activity

Search articles by author

Spotlight

Advertisements