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A multiplex bacterial assay using an element-labeled strategy for 16S rRNA detection

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Abstract

A multiplex bacterial assay method that combines S1 nuclease pretreatment and ICP-MS-based elemental labels is presented in this work. Six intestinal related bacteria were identified at the species level and quantified simultaneously without isolation culturing. This method could be extended to assay a mixed bacterial community for point-of-care diagnosis.

Graphical abstract: A multiplex bacterial assay using an element-labeled strategy for 16S rRNA detection

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Supplementary files

Article information


Submitted
25 Jun 2020
Accepted
13 Aug 2020
First published
17 Aug 2020

Analyst, 2020, Advance Article
Article type
Communication

A multiplex bacterial assay using an element-labeled strategy for 16S rRNA detection

Y. Zhang, G. Sun, Z. Hu, Z. Xing, S. Zhang and X. Zhang, Analyst, 2020, Advance Article , DOI: 10.1039/D0AN01272F

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