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Issue 21, 2020
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A red-emission fluorescent probe for visual monitoring of lysosomal pH changes during mitophagy and cell apoptosis

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Abstract

We presented a novel red-emission fluorescent probe (MSO) for selectively monitoring lysosomal pH fluctuation in living cells. The probe was designed by employing rhodamine B as the off-on pH sensitive moiety owing to the unique spirocycle group and morpholine as the lysosome targetable unit. Based on the H+-induced spirocyclic ring opening process, MSO displayed significant pH sensing properties around 590 nm, with a pKa value of 5.42 and a good linear pH response ranging from 5.00 to 6.00. Besides, the probe possessed other prominent photophysical properties such as good selectivity and excellent photostability as well as low cytotoxicity, together making the red-emission probe more favorable for long-time and real-time imaging in live cells. Furthermore, MSO selectively accumulated into lysosomes and successfully visualized the mitophagy, cell apoptosis and heat shock processes by monitoring the rise of lysosomal pH.

Graphical abstract: A red-emission fluorescent probe for visual monitoring of lysosomal pH changes during mitophagy and cell apoptosis

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Supplementary files

Article information


Submitted
08 Jun 2020
Accepted
06 Aug 2020
First published
06 Aug 2020

Analyst, 2020,145, 7018-7024
Article type
Paper

A red-emission fluorescent probe for visual monitoring of lysosomal pH changes during mitophagy and cell apoptosis

X. Wang, L. Fan, X. Zhang, Q. Zan, W. Dong, S. Shuang and C. Dong, Analyst, 2020, 145, 7018
DOI: 10.1039/D0AN01141J

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