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Single-molecule brightness analysis for the determination of anticancer drug interactions with DNA

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Abstract

Anthracyclines are one of the most studied anticancer drugs approved for medical treatment. The equilibrium constant (K) of the reaction between these drugs with DNA in both in vitro and in vivo experiments lacks consensus. The K values vary from 104 up to 108 M−1, which suggest a 1000-fold error in determining the effective concentration needed to form the drug–DNA complex. Until 2014, only one study by García [J. Phys. Chem. B, 2014, 118, 1288–1295] showed that the binding of anthracycline representative doxorubicin occurs in two reactions. We support this result by brightness analysis at a single molecular level for the four most common anthracyclines: doxorubicin, daunorubicin, epirubicin, and idarubicin.

Graphical abstract: Single-molecule brightness analysis for the determination of anticancer drug interactions with DNA

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Supplementary files

Article information


Submitted
03 Jun 2020
Accepted
18 Jul 2020
First published
20 Jul 2020

This article is Open Access

Analyst, 2020, Advance Article
Article type
Paper

Single-molecule brightness analysis for the determination of anticancer drug interactions with DNA

Y. Zhou, K. Bielec, P. Pasitsuparoad and R. Hołyst, Analyst, 2020, Advance Article , DOI: 10.1039/D0AN01108H

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