Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.



Ultrasensitive detection of butyrylcholinesterase activity based on the inner filter effect of MnO2 nanosheets on sulfur nanodots

Author affiliations

Abstract

Butyrylcholinesterase (BChE) activity is an important index for a variety of diseases. In this work, a “turn-on” assay is proposed based on controlling the inner filter effect (IFE) of MnO2 nanosheets (NSs) on sulfur nanodots (S-dots). The fluorescence of S-dots is effectively quenched by the MnO2 NSs, due to the wide overlap of the emission spectrum of S-dots and absorption spectrum of MnO2 NSs, together with the superior light absorption capability of MnO2 NSs. BChE can catalyze acetylthiocholine and produce thiocholine, which effectively decomposes the MnO2 NSs into Mn2+, resulting in the disappearance of the IFE and recovery of fluorescence of S-dots. Two-stage linear relationships between the ratio of fluorescence intensity and concentration of BChE are observed from 0.05 to 10 and from 10 to 500 U L−1. A limit of detection of 0.035 U L−1 is achieved, which is the best performance so far. The as-proposed assay is robust enough for practical detection in human serum, and it can avoid interference from its sister enzyme (acetylcholinesterase) and glutathione at the micromolar level. The presented results provide a clue for the functionalization of S-dots, and offer a powerful tool as an analytic technique for nanomedicine and environmental science.

Graphical abstract: Ultrasensitive detection of butyrylcholinesterase activity based on the inner filter effect of MnO2 nanosheets on sulfur nanodots

Back to tab navigation

Supplementary files

Article information


Submitted
09 May 2020
Accepted
01 Jun 2020
First published
03 Jun 2020

Analyst, 2020, Advance Article
Article type
Paper

Ultrasensitive detection of butyrylcholinesterase activity based on the inner filter effect of MnO2 nanosheets on sulfur nanodots

T. Li, Y. Gao, H. Li, C. Zhang, Y. Xing, M. Jiao, Y. Shi, W. Li, Y. Zhai and Z. Wang, Analyst, 2020, Advance Article , DOI: 10.1039/D0AN00939C

Social activity

Search articles by author

Spotlight

Advertisements