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Issue 21, 2020
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Aptamer-based strategies for recognizing adenine, adenosine, ATP and related compounds

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Abstract

Adenine is a key nucleobase, adenosine is an endogenous regulator of the immune system, while adenosine triphosphate (ATP) is the energy source of many biological reactions. Selective detection of these molecules is useful for understanding biological processes, biochemical reactions and signaling. Since 1993, various aptamers have been reported to bind to adenine and its derivatives. In addition, the adenine riboswitch was later discovered. This review summarizes the efforts for the selection of RNA and DNA aptamers for adenine derivatives, and we pay particular attention to the specificity of binding. In addition, other molecular recognition strategies based on rational sequence design are also introduced. Most of the work in the field was performed on the classic DNA aptamer for adenosine and ATP reported by the Szostak group. Based on this aptamer, some representative applications such as the design of fluorescent, colorimetric and electrochemical biosensors, intracellular imaging, and ATP-responsive materials are also described. In addition, we critically review the limit of the reported aptamers and also important problems in the field, which can give future research opportunities.

Graphical abstract: Aptamer-based strategies for recognizing adenine, adenosine, ATP and related compounds

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Article information


Submitted
03 May 2020
Accepted
29 Aug 2020
First published
01 Sep 2020

Analyst, 2020,145, 6753-6768
Article type
Critical Review

Aptamer-based strategies for recognizing adenine, adenosine, ATP and related compounds

Y. Li and J. Liu, Analyst, 2020, 145, 6753
DOI: 10.1039/D0AN00886A

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