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DNA-Programmed plasmonic ELISA for the ultrasensitive detection of protein biomarkers

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Abstract

We report a novel DNA-programmed plasmonic enzyme-linked immunosorbent assay (ELISA) for the ultrasensitive detection of protein biomarkers with the naked eye. The DNA-programmed plasmonic assay was based on two enzyme-free and isothermal nucleic acid amplification methods: hybridization chain reaction (HCR) and catalyzed hairpin assembly (CHA). In this study, a biotin-labeled DNA probe was utilized insteand of an enzyme-label probe in well-developed ELISA method. The biotin-labeled DNA probe was able to trigger the HCR and CHA processes, and the products could hybridize with DNA-modified gold nanoparticles (AuNPs) to induce the aggregation of the AuNPs and a color change in the solution. The developed method was able to detect as low as 1 pg mL−1 PSA target with the naked eye. Clinical serum samples demonstrated satisfactory results, indicating that the method is useful for early diagnostics and monitoring curative effects after a medical treatment. The developed method presents a simple and portable platform for ultrasensitive protein detection and has potential for point-of-care (POC) diagnostics in less developed areas.

Graphical abstract: DNA-Programmed plasmonic ELISA for the ultrasensitive detection of protein biomarkers

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Supplementary files

Article information


Submitted
03 Apr 2020
Accepted
09 May 2020
First published
14 May 2020

Analyst, 2020, Advance Article
Article type
Paper

DNA-Programmed plasmonic ELISA for the ultrasensitive detection of protein biomarkers

Y. Cheng, H. Tang, R. Yu and J. Jiang, Analyst, 2020, Advance Article , DOI: 10.1039/D0AN00656D

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