Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.



Portable glucose meter-utilized label-free and washing-free telomerase assay

Author affiliations

Abstract

We herein describe a portable glucose meter (PGM)-utilized label-free and washing-free method for the facile determination of telomerase activity that relies on the kinase-catalyzed cascade enzymatic reaction (KCER) that transduces the telomerase activity to the glucose level. In the sensor, the telomerase that elongates telomere sequences ((TTAGGG)n) from the 3′-terminus of telomerase substrate primer (TSP) consumes deoxynucleoside triphosphate (dNTP), which serves as a phosphate source for KCER promoted by hexokinase and pyruvate kinase. Thus, the presence of telomerase protects KCER from working effectively, resulting in the maintenance of an initial, high glucose level that is readily determined using hand-held PGM. With this strategy, the telomerase activities in various types of cell lines were successfully determined with high sensitivity. Furthermore, the ability of this method to screen candidate inhibitors for telomerase activity was also verified.

Graphical abstract: Portable glucose meter-utilized label-free and washing-free telomerase assay

Back to tab navigation

Supplementary files

Article information


Submitted
03 Apr 2020
Accepted
10 Jun 2020
First published
22 Jun 2020

Analyst, 2020, Advance Article
Article type
Paper

Portable glucose meter-utilized label-free and washing-free telomerase assay

H. Y. Kim, C. Y. Lee, H. Kim, K. S. Park and H. G. Park, Analyst, 2020, Advance Article , DOI: 10.1039/D0AN00655F

Social activity

Search articles by author

Spotlight

Advertisements