Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.



On-chip microfluidic-based electrochemical magneto-immunoassay for procalcitonin determination in plasma from sepsis diagnosed preterm neonates

Abstract

A novel on-chip electrochemical magneto-immunoassay for Procalcitonin (PCT) determination is proposed. The strategy implies the on-line performing of the biorecognition event and the detection onto thin film microfluidic gold electrodes chamber operating at E= −0.20 V (vs Au). The complete assay was performed in less than 15 minutes using only 25 µL of sample, covering the entire spectrum of relevant clinically PCT concentrations in sepsis diagnosis and with a limit of detection and quantification of 0.02 ng/mL and 0.05 ng/mL, respectively (sepsis diagnosis threshold: 0.5 ng/mL). The on-chip electrochemical magneto-immunoassay provided excellent results in the analysis of very unique samples coming from preterm neonates with sepsis suspicion, in which sample volume is hardly available. These characteristics fulfill the POCT requirements for PCT determination in the whole relevant clinical concentrations. Because of the high clinical relevance and the important role of PCT in sepsis, this approach opens new perspectives for the sepsis diagnosis and therapy guidance using low volume samples.

Back to tab navigation

Article information


Submitted
30 Mar 2020
Accepted
20 May 2020
First published
22 May 2020

Analyst, 2020, Accepted Manuscript
Article type
Paper

On-chip microfluidic-based electrochemical magneto-immunoassay for procalcitonin determination in plasma from sepsis diagnosed preterm neonates

A. Molinero-Fernández, M. Á. López and A. Escarpa, Analyst, 2020, Accepted Manuscript , DOI: 10.1039/D0AN00624F

Social activity

Search articles by author

Spotlight

Advertisements