On-chip microfluidic-based electrochemical magneto-immunoassay for procalcitonin determination in plasma from sepsis diagnosed preterm neonates
A novel on-chip electrochemical magneto-immunoassay for Procalcitonin (PCT) determination is proposed. The strategy implies the on-line performing of the biorecognition event and the detection onto thin film microfluidic gold electrodes chamber operating at E= −0.20 V (vs Au). The complete assay was performed in less than 15 minutes using only 25 µL of sample, covering the entire spectrum of relevant clinically PCT concentrations in sepsis diagnosis and with a limit of detection and quantification of 0.02 ng/mL and 0.05 ng/mL, respectively (sepsis diagnosis threshold: 0.5 ng/mL). The on-chip electrochemical magneto-immunoassay provided excellent results in the analysis of very unique samples coming from preterm neonates with sepsis suspicion, in which sample volume is hardly available. These characteristics fulfill the POCT requirements for PCT determination in the whole relevant clinical concentrations. Because of the high clinical relevance and the important role of PCT in sepsis, this approach opens new perspectives for the sepsis diagnosis and therapy guidance using low volume samples.