Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.



Colorimetric discriminatory array for detection and discrimination of antioxidants based on HAuCl4/3,3′,5,5′-tetramethylbenzidine

Author affiliations

Abstract

Here, we report a simple but effective nose/tongue-mimic sensor array based on HAuCl4/3,3′,5,5′-tetramethylbenzidine (TMB) for colorimetric discrimination and determination of antioxidants. Two concentrations of HAuCl4 were employed as receptor units to construct the colorimetric sensor array. The sensing strategy is based on the fact that HAuCl4 with different concentrations (0.08 and 0.03 mM) could oxidize TMB to oxidized TMB (oxTMB), resulting in a blue and green color solution, respectively, corresponding to an absorption peak centered at 440 nm and 657 nm. However, the presence of different antioxidants could cause the reduction in HAuCl4, leading to the fading of the blue and green color and the decrease in the absorbance at 440 nm and 657 nm to varying degrees. Based on the above phenomena, by taking advantage of linear discriminant analysis (LDA), five antioxidants (i.e. ascorbic acid (AA), melatonin (MT), uric acid (UA), tannic acid (TCA), and glycine (Gly)) at five concentrations (200, 120, 60, 20, and 1 nM) were successfully discriminated both in buffer and serum. More importantly, this approach is simple, fast, and without the use of any nanomaterials.

Graphical abstract: Colorimetric discriminatory array for detection and discrimination of antioxidants based on HAuCl4/3,3′,5,5′-tetramethylbenzidine

Back to tab navigation

Supplementary files

Article information


Submitted
30 Mar 2020
Accepted
09 Jun 2020
First published
10 Jun 2020

Analyst, 2020, Advance Article
Article type
Paper

Colorimetric discriminatory array for detection and discrimination of antioxidants based on HAuCl4/3,3′,5,5′-tetramethylbenzidine

B. Liu, Y. Chen, M. Zhang, Z. Chen and X. Zuo, Analyst, 2020, Advance Article , DOI: 10.1039/D0AN00617C

Social activity

Search articles by author

Spotlight

Advertisements