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A novel strategy for rhodamine B-based fluorescent probes with a selective glutathione response for bioimaging in living cells

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Abstract

The aim of this study was to overcome the reported shortcomings of the glutathione (GSH) detection of rhodamine-based fluorescent probes, such as poor selectivity to thiol groups and reversible unstable covalent binding with the thiol groups. Here, we have developed a simple and specific fluorescent probe based on rhodamine B, which can be used to selectively detect GSH in solution and perform bioimaging in living cells. This design strategy uses a specific reaction between allenamide and the GSH thiol group, which proceeds rapidly in a phosphate buffer/tetrahydrofuran (PBS/THF) mixture with specific selectivity, and forms a stable and irreversible conjugate. The combined simplicity and specificity of the recognition process enables it to serve as a fluorescent probe for detecting GSH level changes in living cells. Such a new recognizing strategy thus may open a new window for its further application in detecting GSH levels both in vitro and in vivo.

Graphical abstract: A novel strategy for rhodamine B-based fluorescent probes with a selective glutathione response for bioimaging in living cells

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Article information


Submitted
24 Mar 2020
Accepted
25 Apr 2020
First published
27 Apr 2020

Analyst, 2020, Advance Article
Article type
Paper

A novel strategy for rhodamine B-based fluorescent probes with a selective glutathione response for bioimaging in living cells

Z. Li, W. Xiong, X. He, X. Qi, F. Ding and J. Shen, Analyst, 2020, Advance Article , DOI: 10.1039/D0AN00582G

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