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An innovative blood plasma separation method for a paper-based analytical device using chitosan functionalization

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Abstract

This study describes a microfluidic paper-based analytical device (μPAD) for separating plasma from whole blood and measuring glucose concentration. A two-dimensional μPAD was fabricated by wax printing, using chromatographic paper and was functionalized by the incorporation of chitosan into the plasma separation zone. Red blood cells were isolated from whole blood in the plasma separation zone consisting of a chitosan polymer structure and a wax barrier. The separated plasma, with buffer, was injected into the test zone to cause a color change. The colorimetric results were digitized with a scanner, and the concentration was calculated using a calibration curve. This μPAD, which separated the plasma from whole blood without a separation membrane, will be useful for constructing point-of-care testing devices that detect analytes in small sample volumes.

Graphical abstract: An innovative blood plasma separation method for a paper-based analytical device using chitosan functionalization

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Article information


Submitted
13 Mar 2020
Accepted
10 Jun 2020
First published
16 Jun 2020

Analyst, 2020, Advance Article
Article type
Paper

An innovative blood plasma separation method for a paper-based analytical device using chitosan functionalization

D. Kim, S. Kim and S. Kim, Analyst, 2020, Advance Article , DOI: 10.1039/D0AN00500B

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