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Productive screening of single aptamers with ddPCR

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Abstract

Antibodies have now been widely used for clinical treatment of a number of tumors. However, there are serious problems associated with antibody therapy, such as potential interactions of antibodies with the immune system as well as long production cycles. Recently, aptamers have been found to function similar to antibodies in terms of affinity and specificity to certain proteins and are attracting much attention for their low immunogenicity, easy chemical synthesis, and efficient penetration into tissues due to their small size. However, how to access high affinity and selectivity aptamers efficiently for further analysis is still open to be resolved. Herein, an aptamer discovery method that combines the continuous flow ddPCR technology with cytometer sorting of beads is reported, such that we have obtained DNA aptamers binding specifically to PD-1 with an affinity of over 60-fold higher than that for the best-reported method.

Graphical abstract: Productive screening of single aptamers with ddPCR

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Supplementary files

Article information


Submitted
06 Mar 2020
Accepted
04 May 2020
First published
05 May 2020

Analyst, 2020, Advance Article
Article type
Communication

Productive screening of single aptamers with ddPCR

J. Chen, J. Wang, Z. Luo, X. Fang, L. He, J. Zhu, Z. Qurat ul ain, J. He, H. Ma, H. Zhang, M. Liu and L. He, Analyst, 2020, Advance Article , DOI: 10.1039/D0AN00460J

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