Nano-crystalline cellulose coated magnetic nanoparticle used for glycoprotein’s affinity adsorption
A new core-shell structured nanomaterial based on Fe3O4 nanoparticle and 2, 3-dialdehyde nanocrystalline cellulose (DAC) coating and its high efficiency in preconcentration of glycoproteins were described in this work. DAC was obtained after periodate oxidation of the nanocrystalline cellulose to form aldehyde groups and then Fe3O4 nanoparticles was coated with DAC, which was further attached with 4-aminophenylboronic acid (PBA) to form PBA-functionalized magnetic core-shell structured materials (Fe3O4@DAC-PBA). The oxidation of cellulose and production of enough amounts of aldehyde group sites was essential to the preparation of (Fe3O4@DAC-PBA) used for glycoproteins’ affinity adsorption, because aldehyde groups on DAC allowed the DAC to be both attached onto Fe3O4 nanoparticles and bound with PBA, which was active in forming complex with glyco sites in glycoproteins. Moreover, the preconcentration properties of Fe3O4@DAC-PBA through PBA adsorption can be pH-triggered without disassembly of the structures, so the developed Fe3O4@DAC-PBA can be efficiently prepared to provide a promising affinity material for affinity adsorption and purification of glycoproteins.