Establishment of a universal and sensitive plasmonic biosensor platform based on the hybridization chain reaction (HCR) amplification induced by a triple-helix molecular switch
Herein, we established a universal and sensitive plasmonic sensing strategy for biomolecules assay by coupling the hybridization chain reaction (HCR) strategy and a triple-helix molecular switch. Upon the recognition of the target, a single-stranded DNA as a universal trigger (UT) was released from the triple-helix molecular switch (THMS). Thus, the HCR process can be triggered between two hairpins M1 and M2, resulting in the aggregation of gold nanoparticles (AuNPs) via the hybridization between the tail sequence on M1 (or M2) and DNA-AuNPs tag probe and accompanying a dramatic absorbance change at 521 nm. More specifically, the strategy, which was conducted by the introduction of target‐specific recognition of THMS without changing the based on target changes, enables simple design for multiple target detection. By taking advantage of the THMS, this strategy could enable stable and sensitive detection of a variety of targets involving nucleic acids, small molecules and proteins, which may possess great potential for practical application.