Jump to main content
Jump to site search


Received 00th January 20xx, Accepted 00th January 20xx DOI: 10.1039/x0xx00000x A dendritically amplified fluorescence signal probe on SiO2 microspheres for ultrasensitive detection of mercury ions

Abstract

In this work, a new kind of dendritically amplified fluorescence signal probe on SiO2 microspheres were controllably fabricated by terminal deoxynucleotidyl transferase (TdT)-catalyzed incorporation of nucleotides combined with bio-bar-code (BBC) amplification for ultrasensitive detection of Hg2+. The thymine T-Hg2+-T hairpin structure was firstly formed and further initiated the strand-displacement-amplification (SDA) reaction, generating mimic target (MT). MT hybridized with capture probe 1 (c1) on SiO2 microspheres, and the 3′-hydroxyl (OH) termini of MT initiated TdT-based DNA extension, producing abundant poly-Guanine sequence (G1). Then G1 hybridized with capture probes (C2) with poly-Cytosine (C) to assemble multiple C2/reporter probe-AuNPs onto the SiO2 microspheres. The reporter DNA further initiated TdT-based extension with poly T sequence (T1) to link large numbers of signal probes, which generated very high fluorescence signal for ultrasensitive detection of target Hg2+. This TdT-based signal amplification method coupled with SDA exhibits extraordinary sensitivity for Hg2+ assay with a limit down to 1.0 aM. The proposed highly sensitive fluorescence strategy holds great potential for detecting targets in environmental and biological fields.

Back to tab navigation

Supplementary files

Article information


Submitted
21 Jan 2020
Accepted
10 Feb 2020
First published
10 Feb 2020

Analyst, 2020, Accepted Manuscript
Article type
Paper

Received 00th January 20xx, Accepted 00th January 20xx DOI: 10.1039/x0xx00000x A dendritically amplified fluorescence signal probe on SiO2 microspheres for ultrasensitive detection of mercury ions

C. Li, Y. Zhang, Q. Cai, G. Jie and C. li, Analyst, 2020, Accepted Manuscript , DOI: 10.1039/D0AN00158A

Social activity

Search articles by author

Spotlight

Advertisements