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Neutral DNA–avidin nanoparticles as ultrasensitive reporters in immuno-PCR

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Abstract

We have developed an immuno-PCR based diagnostic platform which couples detection antibodies to self-assembled, ultra-detectable DNA–avidin nanoparticles stabilized with poly(ethylene glycol) to link DNA amplification to target protein concentration. Electrostatic neutralization and cloaking of the PCR-amplifiable DNA labels by avidin and PEG coating reduces non-specific “stickiness” and enhances assay sensitivity. We further optimized the detectability of the nanoparticles by incorporating four repeats of a unique synthetic DNA PCR target into each nanoparticle. Using human chorionic gonadotropin hormone (hCG) as a model analyte, this platform was able to quantitate the target hCG protein in femtomolar concentrations using only standard laboratory equipment.

Graphical abstract: Neutral DNA–avidin nanoparticles as ultrasensitive reporters in immuno-PCR

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Supplementary files

Article information


Submitted
17 Jan 2020
Accepted
26 May 2020
First published
05 Jun 2020

Analyst, 2020, Advance Article
Article type
Paper

Neutral DNA–avidin nanoparticles as ultrasensitive reporters in immuno-PCR

D. Chavan, H. Chen, M. Crum, B. Vu, M. Safari, M. Smith, P. Vekilov, J. C. Conrad, K. Kourentzi and R. C. Willson, Analyst, 2020, Advance Article , DOI: 10.1039/D0AN00134A

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