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A traceless clean-up method coupled with gas chromatography and mass spectrometry for analyzing polycyclic aromatic hydrocarbons in complex plant leave matrices

Abstract

This study developed a traceless clean-up method combining solid phase extraction (SPE) with gas purge–microsyringe extraction (GP-MSE) to purify sample extracts for the determination of polycyclic aromatic hydrocarbons (PAHs) in plant leaves. SPE exhibited good purification performance for the removal of polar lipid, while the GP-MSE technique effectively eliminate low-volatile lipid hence realizing zero damage to instrument, and significantly improve the peak tailings. After ultrasonic extraction, the combined two-step clean-up procedure successfully removed over 99% of lipids from nineteen types of tree leaves, and PAHs from tree leaves were determined by GC-MS. The relative standard deviation (RSD) for intra-day (n=3) and inter-day (n=3) analysis of PAHs from spiked willow samples were 0.8% – 12.1% and 4.7% – 15.3%, respectively. The recoveries of PAHs from spiked willow extracts ranged from 74 to 90%, with an average of 86%. The method detection limit (MDL) of PAHs in tree leaves ranged from 0.1 to 4.9 ng g-1 dry weight. In conclusion, the clean-up method in this study realized the analysis of PAHs from plant leaves with high accuracy, sensitivity and reproducibility. Most importantly, the two-step purification method significantly minimizes damage to the GC-MS system particularly on the column and ion source, which is beneficial to ensure continuous analysis for a large number of samples with good performance.

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Supplementary files

Article information


Submitted
17 Jan 2020
Accepted
19 Feb 2020
First published
19 Feb 2020

Analyst, 2020, Accepted Manuscript
Article type
Paper

A traceless clean-up method coupled with gas chromatography and mass spectrometry for analyzing polycyclic aromatic hydrocarbons in complex plant leave matrices

X. Jin, H. Y. Kaw, H. Li, Z. Wang, J. Zhao, X. Piao, D. Li, D. Jin and M. He, Analyst, 2020, Accepted Manuscript , DOI: 10.1039/D0AN00128G

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