A mitochondria-targeted fluorescence probe for the detection of endogenous SO2 derivatives in living cells
A unique fluorescence probe (ZACA) for the monitoring of SO2 derivatives was developed from coumarin and benzoindoles based on FRET and ICT platform. ZACA exhibited active emission signal, large Stokes shift, wide emission window distance, high photo stability. ZACA possessed many advantages in the ratiometric detection of HSO3-/SO32- including low detection limit, high selectivity and sensitivity. Importantly, ZACA had been successfully applied to the ratiometric detection of endogenous HSO3-/SO32- in living cells with excellent cellular imaging capability (1 μM) and mitochondria-targeted ability (co-localization coefficient 0.91).