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Rapid quantification of prion proteins using resistive pulse sensing

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Abstract

Prion diseases are a group of fatal transmissible neurological conditions caused by the change in conformation of intrinsic cellular prion protein (PrPC). We present a rapid assay using aptamers and resistive pulse sensing, RPS, to extract and quantify PrPC from complex sample matrices. We functionalise the surface of superparamagnetic beads, SPBs, with a DNA aptamer. First SPB's termed P-beads, are used to pre-concentrate the analyte from a large sample volume. The PrPC protein is then eluted from the P-beads before aptamer modified sensing beads, S-beads, are added. The velocity of the S-beads through the nanopore reveals the concentration of the PrPC protein. The process is done in under an hour and allows the detection of picomol's of protein.

Graphical abstract: Rapid quantification of prion proteins using resistive pulse sensing

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Article information


Submitted
10 Jan 2020
Accepted
11 Feb 2020
First published
12 Feb 2020

Analyst, 2020, Advance Article
Article type
Paper

Rapid quantification of prion proteins using resistive pulse sensing

M. J. Healey, M. Sivakumaran and M. Platt, Analyst, 2020, Advance Article , DOI: 10.1039/D0AN00063A

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