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Issue 7, 2020
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Rapid quantification of prion proteins using resistive pulse sensing

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Abstract

Prion diseases are a group of fatal transmissible neurological conditions caused by the change in conformation of intrinsic cellular prion protein (PrPC). We present a rapid assay using aptamers and resistive pulse sensing, RPS, to extract and quantify PrPC from complex sample matrices. We functionalise the surface of superparamagnetic beads, SPBs, with a DNA aptamer. First SPB's termed P-beads, are used to pre-concentrate the analyte from a large sample volume. The PrPC protein is then eluted from the P-beads before aptamer modified sensing beads, S-beads, are added. The velocity of the S-beads through the nanopore reveals the concentration of the PrPC protein. The process is done in under an hour and allows the detection of picomol's of protein.

Graphical abstract: Rapid quantification of prion proteins using resistive pulse sensing

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Supplementary files

Article information


Submitted
10 Jan 2020
Accepted
11 Feb 2020
First published
12 Feb 2020

Analyst, 2020,145, 2595-2601
Article type
Paper

Rapid quantification of prion proteins using resistive pulse sensing

M. J. Healey, M. Sivakumaran and M. Platt, Analyst, 2020, 145, 2595
DOI: 10.1039/D0AN00063A

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