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Issue 4, 2020
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Ratiometric fluorescence method for ctDNA analysis based on the construction of a DNA four-way junction

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Abstract

We present a novel ratiometric fluorescent biosensor for ctDNA analysis based on the construction of a DNA four-way junction (FWJ). Three fuel strands for the FWJ are firstly designed and prepared. Another essential strand for the formation of the structure is the DNA product generated from target ctDNA initiated strand displacement amplification. With the transformation of the DNA structure, the FRET states of two fluorophores change and the ratiometric fluorescence response can be recorded to indicate the level of the initial ctDNA. The proposed method also has excellent capability to discriminate mismatches and shows potential practical utility for clinical samples.

Graphical abstract: Ratiometric fluorescence method for ctDNA analysis based on the construction of a DNA four-way junction

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Supplementary files

Article information


Submitted
08 Jan 2020
Accepted
26 Jan 2020
First published
27 Jan 2020

Analyst, 2020,145, 1174-1178
Article type
Communication

Ratiometric fluorescence method for ctDNA analysis based on the construction of a DNA four-way junction

G. Liu, X. Ma, Y. Tang and P. Miao, Analyst, 2020, 145, 1174
DOI: 10.1039/D0AN00044B

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