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A sensitive method for the quantification of short-chain fatty acids by benzyl chloroformate derivatization combined with GC-MS

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Abstract

Short-chain fatty acids (SCFAs) were identified as critical markers in the diagnosis of chronic and metabolic diseases, but a sensitive and stable method to determine SCFAs in feces is a challenge for analysts due to the high volatility. Herein, a sensitive and accurate method to determine SCFAs adopting precolumn derivatization coupled with gas chromatography-mass spectrometry (GC-MS) has been developed. Benzyl chloroformate (BCF) was chosen as the reaction reagent and emulsified derivatization was applied to homogenize the reaction system. Higher sensitivity, wider application and satisfactory derivatization efficiency were obtained using the developed method. An excellent method validation showed a good linearity ranging from 0.9947 to 0.9998. At the same time, the intra-day and inter-day precision were achieved in the range of 0.56% to 13.07%. The lower limits of detection of all target analytes varied from 0.1 to 5 pg. The recovery ranged from 80.87% to 119.03%, and storage stability under three different conditions was also determined. This method was also successfully applied to the analysis of SCFAs in mice fecal samples to illustrate the significant differences between normal and type 2 diabetes mellitus mice.

Graphical abstract: A sensitive method for the quantification of short-chain fatty acids by benzyl chloroformate derivatization combined with GC-MS

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Article information


Submitted
02 Jan 2020
Accepted
06 Feb 2020
First published
10 Feb 2020

Analyst, 2020, Advance Article
Article type
Paper

A sensitive method for the quantification of short-chain fatty acids by benzyl chloroformate derivatization combined with GC-MS

M. Li, R. Zhu, X. Song, Z. Wang, H. Weng and J. Liang, Analyst, 2020, Advance Article , DOI: 10.1039/D0AN00005A

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