Application of a Y-type-DNA-functionalized nanogold probe featuring specific telomerase recognition and doxorubicin release in cancer cells
A Y-type-DNA-functionalized nanogold probe was synthesized to identify telomerase and trigger drug release in cancer cells. This system involved a DNA-functionalized nanogold probe centering on gold nanoparticles with dense modification of Y-type DNA molecular beacons on the surface. The Y-type DNA molecular beacon consisted of the telomerase primers (TP), FAM-labeled single-strand DNA (Mismatch-DNA), and single- strand DNA of two templates (Linker-DNA1,Linker-DNA2). Doxorubicin (Dox), an anti-cancer drug molecule, was inserted into DNA double strands. When telomerase existed, TP was extended, Mismatch-DNA was released, leading to emition of fluorescent light from the FAM while releasing Dox. This probe specifically detected cancer cells and did not affect normal cells. This drug delivery system will reduce tumour size and cause minimal injury to normal tissues.